I am trying to culture stem like cells from adult pancreas but i think something is missing in my media.
I am using the well described method for growing neural stem cells. hyclone dmem without serum, fgf, egf, heparin, n2, b27, neuro basal media and in low attachemnt conditions. does anyone know another strategy to culture adult pancreatic stem cells?
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sphere formation assay - pancreas adult stem cells
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