What would vortexing competent cells do during the transformation process (i.e. thawing from -80, after adding plasmid DNA etc)?
Will it lyse the cells?
0
1 reply to this topic
#2
leelee
-
- Active Members
-
- 631 posts
Veteran
49
Excellent
Excellent
Posted 07 December 2011 - 07:53 AM
Competent cells are fragile, so yes I think that vortexing them will kill a great deal of them. When I add my DNA prior to transformation, I mix gently with a large bore pipette, or invert several times, then incubate the cells on ice for about 15-30 min.
