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YPD + Zeo agar plates

agar plate zeo ypd

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2 replies to this topic

#1 jenchung3

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Posted 05 December 2011 - 05:49 PM

I made several YPD + Zeo plates today, but I think I may have added 2/3 of the Zeo that I was supposed to add. Can I still use the YPD + Zeo plates, or will I need to make new ones?

Is there a simple test I can run to check if the plates are okay to use?

Edited by jenchung3, 05 December 2011 - 05:50 PM.


#2 phage434

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Posted 05 December 2011 - 07:33 PM

Plate out wild type and zeo resistant strains on one of your plates. The wt should not grow, the resistant ones should.

#3 lamaksha77

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Posted 05 December 2011 - 08:39 PM

You could also look up the minimal bactericidal concentration (MBC) for zeocin for your wild-type strain, and then check if the zeo concentration in your plates is just above that value...if it is you should be mostly fine.





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