2 replies to this topic

[jenchung3]

I made several YPD + Zeo plates today, but I think I may have added 2/3 of the Zeo that I was supposed to add.  Can I still use the YPD + Zeo plates, or will I need to make new ones?

Is there a simple test I can run to check if the plates are okay to use?

Edited by jenchung3, 05 December 2011 - 05:50 PM.

[phage434]

Plate out wild type and zeo resistant strains on one of your plates.  The wt should not grow, the resistant ones should.

[lamaksha77]

You could also look up the minimal bactericidal concentration (MBC) for zeocin for your wild-type strain, and then check if the zeo concentration in your plates is just above that value...if it is you should be mostly fine.