maverick3006, on 06 December 2011 - 12:20 AM, said:
Hi all.......here are some pix of these intruders. These have been taken 12 hours after changing the media in a culture maintained for 4 days. They look horrible, don't they??
45 and 46 are at 10X mag and 50 at 40X mag.
Uncle Rhombus- your suggestion about this being something to do without scleroderma itself makes sense to me. So, I am going to culture fibroblasts from healthy volunteers in a day or two and hope that they donot show any contamination.
In the meantime, based on these pic can anyone help me?? Can these cultures be salvaged or should I discard them?
Dear Maverick 3006
They are definetely contaminated and need to be chucked away. It is hard to tell if it is a yeast but I could not make out one contaminant that had a "budding" morphology, which is indicative of yeast infections.
The problem with "infections" in general is that the bug will release bacterial wall contents into the culture and this WILL affect the skin fibroblasts. Just the presence of bugs may affect the cells directly. If you try and use an antibiotic to kill the infection ...then this WILL definetely affect the fibroblasts.
Your idea of preparing fibroblasts from so called "healthy" patients is a good one.
Generally speaking fungal contaminations come from the local environment i.e. the CO2 incubator
Yeast and other bacterial infections can come from poor aseptic technique as leelee quite correctly states.