Cell lysate turbidity upon long storage
Posted 04 December 2011 - 08:57 PM
Iam working on mammalian cell lines to study their apoptosis, for that iam treating cells (5million roughly)with drugs to induce apoptosis later using Mper to lyse the cells.these cell lysate stored at -20oC.after a week or so i started observing tubidity in samples.why? where is the problem.people suggest me what should i do .
Posted 09 December 2011 - 03:32 PM
Posted 28 December 2011 - 03:54 PM
Mper Invitrogen is the lysis buffer i used and in that protease inhibitor sigma added. before storage i spun samples at 13,000RPM for 10mins.
Posted 28 December 2011 - 07:35 PM