Is there a generally accepted rule of thumb for how to feed cells for a weekend? If you normally feed every 2 days can you just give them more media on Friday (and how much more)? Does it make a difference if there are or aren't growth factors in your media for whatever cell types you're working on at the time?
Thanks.
Submit your paper to J Biol Methods today!
Splitting or feeding cells for the weekend
Started by assembler01, Dec 02 2011 10:45 AM
tissue culture cell feed
1 reply to this topic
#2
bob1
-
- Global Moderators
-
- 5,115 posts
Thelymitra pulchella
327
Excellent
Excellent
Posted 02 December 2011 - 02:09 PM
It will depend on the cell line, but I usually find that most of the common cell lines will easily last over the weekend on whatever medium they are in. Adding more medium would work to some extent, though often it is not about adding fresh nutrients, but removing waste products.
Some growth factors are very labile, so they may well degrade over a day or so.
Some growth factors are very labile, so they may well degrade over a day or so.
Also tagged with one or more of these keywords: tissue, culture, cell, feed
 |
Protocols and Techniques Forums →
Cell Biology →
ATP Formation and metabolic pathways based on Cellular RespirationStarted by caters, 11 Feb 2014  ATP, cell, cellular respiration and 2 more...
|
|
|
|
Protocols and Techniques Forums →
General Lab Techniques →
Do S2 cells normally have small 1-2um objects floating in culture?Started by fruitfly1, 07 Feb 2014 culture, drosophila, cell, S2 and 1 more...
|
|
|
||
Protocols and Techniques Forums →
Microbiology →
Problem in Cell ViewingStarted by Celz, 06 Feb 2014 microbiology, cell, microscope
|
|
|
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
Lymphocyte Cell LineStarted by jerryshelly1, 16 Jan 2014 lymphocyte, cell line, culture
|
|
|
||
 Answered
Protocols and Techniques Forums →
Tissue and Cell Culture →
Cell line identity (HEK293-E)Started by Osibisa, 14 Jan 2014 HEK293, cell, identity
|
|
|
