2 replies to this topic

[Mati]

Hi guys i am using HEK 293 cells but i found some black particles moving in my cell culture even some are attached to the surface of the cells.
Can i use these cells for transfection??? I have attached some pics of these particles plz let me know if you have soem idea??

[bob1]

See here: http://www.protocol-...-helpful-paper/

[Gangwolf]

Take the supernatant containing the black dots and "culture" this in a cell culture incubator separately from your eukaryotic cells. If these dots are proliferating then I would say it is bacterial contamination.
Upon any bacterial contamination, discard your contaminated cells as they would give you inconsistant experimental results. In addition, contaminated cells might be very difficult to transfect.

Good luck