Hi, I am interested in expressing a C-terminal fragment (~800aa) of a protein, but I want to keep the signal peptide (N-terminal, ~20aa) with it.
How should I make the recombinant protein? The signal peptide is ~60bp long, so it is hard to directly include in the 5'-primer?
Would someone please help me with it?
Thanks a lot!
0
how to express a protein fragment together with the signal peptide
Started by joy123, Dec 01 2011 02:53 PM
recombinant protein signal peptide
No replies to this topic
Also tagged with one or more of these keywords: recombinant protein, signal peptide
Protocols and Techniques Forums →
SDS-PAGE and Western Blotting →
Can I improve my primary antibody?Started by Guest_kmobri08_* , 28 May 2012  serum, primary antibody, purify and 1 more...
|
|
|
||
Protocols and Techniques Forums →
Protein Expression and Purification →
E. coli cells refuse to grow!Started by Guest_Kate Mittendorf Pereira_* , 15 Mar 2012 Protein expression and 3 more...
|
|
|
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
Problem with expression of recombinant proteinStarted by Guest_Dr.Kurva_* , 02 Dec 2011 recombinant protein and 2 more...
|
|
|
