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how to express a protein fragment together with the signal peptide

recombinant protein signal peptide

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#1 joy123



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Posted 01 December 2011 - 02:53 PM

Hi, I am interested in expressing a C-terminal fragment (~800aa) of a protein, but I want to keep the signal peptide (N-terminal, ~20aa) with it.

How should I make the recombinant protein? The signal peptide is ~60bp long, so it is hard to directly include in the 5'-primer?

Would someone please help me with it?

Thanks a lot!

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