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horizontal smear?


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2 replies to this topic

#1 rick112

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Posted 01 December 2011 - 11:28 AM

hi

what could be the reasons for horizontal smearing in SDS-PAGE??

#2 mdfenko

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Posted 01 December 2011 - 11:42 AM

in general, salts, lipids, detergents, etc in the sample.

a picture and more information would help to determine the specific cause.
talent does what it can
genius does what it must
i do what i get paid to do

#3 science noob

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Posted 05 December 2011 - 11:06 PM

hi

what could be the reasons for horizontal smearing in SDS-PAGE??


Horizontal smearing would most likely be due to too much sample loaded in the wells causing side-by-side bands to merge?




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