Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

question about mRNA isolation by Triszol

mRNA trizol stem cell

  • Please log in to reply
No replies to this topic

#1 allenandthomas



  • Members
  • Pip
  • 1 posts

Posted 01 December 2011 - 12:57 AM

I am a newbie in mRNA isolation.
I have a question to ask and hope someone can help me out.
I was using Trisure ( which is similar to trizol) to isolate mRNA from inslin producing cell ( which is differentiated from stem cell)
Following are my steps:
Collect cell( clusters) à add Trisure à grind the clusters à adding chloroform à centrifuge at 12000rpm for 15 min at 4 ºC
Then here comes my question. I saw something weird( white stuff) are floating in the upper layer. Does anyone know what it might be and how to avoid it happened again. Does it mean that I didn’t homogenize clusters well?
Thank you in advance.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.