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how to find 16srRNA sequences?

Started by joy123, Nov 30 2011 07:03 PM

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3 replies to this topic

#1 joy123

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Posted 30 November 2011 - 07:03 PM

I want to do test 16srRNA of certain bacteria.

Where can I find the sequences of bacteria 16srRNA? Is there special database?

And are there special tricks for designing their primers?

Thanks very much!

#2 joy123

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Posted 30 November 2011 - 07:05 PM

I will do Real-Time PCR.

#3 phage434

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Posted 30 November 2011 - 07:42 PM

Check this link:
http://openwetware.o..._identification

#4 pcrman

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Posted 30 November 2011 - 08:34 PM

You can also search NCBI Gene database to find the gene for your organism, and then the actual nucleic acid sequence
http://www.ncbi.nlm..../?term=16s rrna
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