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how to make a recombinant protein with signal peptide


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#1 joy123

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Posted 30 November 2011 - 06:40 PM

I want to express a part of a protein, but I want to include the signal peptide.

What is the general procedure of making a recombinant protein, with addition of a N-term signal peptide? Should I include the peptide coding sequence in the 5'-primer? But it is very long (~60bp).

Thanks a lot!

#2 protolder

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Posted 30 November 2011 - 11:17 PM

Hola, the general procedure is subcloning your protein in a vector which will contains signal peptide. There are some in the pET system for bacteria and in any other expression system that you choice as baculovirus for instance that I know. Buena suerte




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