Hi,
I’m isolating small RNA from total RNA using a 15% TBE-urea PAGE gel (following the small RNA Illumina protocol). I’m obtaining a high smallRNA concentration (around 200ng/ul) but the A260/280 reads are low (1.26-1.57) so I don’t know if I should go ahead with the construction of the library. Is there any method to purify my small RNA and remove the protein contamination before starting the libraries?
Any help will be greatly appreciated!
0
purify small RNA
Started by cmdiez, Nov 30 2011 12:58 PM
siRNA RNA extraction contamination purification
No replies to this topic
Also tagged with one or more of these keywords: siRNA, RNA, extraction, contamination, purification
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