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purify small RNA

siRNA RNA extraction contamination purification

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#1 cmdiez



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Posted 30 November 2011 - 12:58 PM

I’m isolating small RNA from total RNA using a 15% TBE-urea PAGE gel (following the small RNA Illumina protocol). I’m obtaining a high smallRNA concentration (around 200ng/ul) but the A260/280 reads are low (1.26-1.57) so I don’t know if I should go ahead with the construction of the library. Is there any method to purify my small RNA and remove the protein contamination before starting the libraries?
Any help will be greatly appreciated!

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