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RNA extraction from paraffin embedded tissue

RNA extraction

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#1 tapi

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Posted 30 November 2011 - 05:09 AM

Hi everybody. I would like to know if it´s possible to extract RNA from tissue embedded in paraffin. Is there any protocol for this?

Thanks to all

#2 Trof

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Posted 30 November 2011 - 09:31 AM

There are several kits from ABI/Invitrogen, Roche, QIAGEN and so on.
But I was just looking at this paper yesterday, where is a protocol for RNA isolation from FFPE by classic phenol:chlorophorm extraction.
Paraffin-based 6-gene model predicts outcome in diffuse large B-cell lymphoma patients treated with R-CHOP

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#3 tapi

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Posted 30 November 2011 - 11:21 AM

In my lab we have old material embedded in paraffin and also a lot of material en EtOH 100% at -20º that was going to be used for histology. It would be possible to extract also from material in EtOH? Do you think the RNA could be degraded? I think thath because the tissue is fixed, maybe it could be intact





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