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Voltage/Time Recommendations for BioRad Criterion Blotter


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#1 scichem

scichem

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Posted 29 November 2011 - 12:20 PM

I'm using the BioRad Criterion Blotter (plate electrodes), and I was wondering if you had suggestions for voltage/time conditions. The manufacturer suggests 100V for 30 minutes, but I am wanting to transfer a small protein (10-15 kDa) so I was wondering if I need to reduce the time or voltage to prevent my protein from blowing through the membrane.

Thanks!




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