Hi everyone! I have a question about immunofluorescence. I want to detect protein X and my Primary antibody is: Guinea Pig anti -X . The problem is that the only anti Guinea Pig secondary antibody I have is: Goat anti-Guinea Pig IgG HRP conjugate (I use it for WB). So I would like to know if I can do an Immunofluorescence using that 2 antibodies and an anti goat-FITC (like a sandwich). So anybody knows if I can do that? Has it been done before?
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immunofluorescence using 3 antibodies like a sandwich
Started by osho, Nov 29 2011 12:11 PM
Immunofluorescence antibodies sandwich
3 replies to this topic
#2
BioMiha
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Posted 30 November 2011 - 11:08 PM
Although I haven't tried this exactly, I think it should work.
#3
bob1
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Posted 02 December 2011 - 02:27 PM
It should work; a lot of the immunocytochemisty systems use a 3 antibody set up for detection.
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