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total RNA Extraction gel control

RNA gel RNA bands

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#1 maegmor



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Posted 29 November 2011 - 12:32 AM

I was trying to isolate total RNA from tumor samples and when i ran the extracted samples on gel, i saw 4 bands instead of classic 3 bands which you get to see after total RNA isolation. I believe that the presence of a 4. band is due to the existence of genomic DNA along with total RNA, since i didnt use DNase and some of the samples show up as smears, which i thought is a result of degradation. But i would like to hear your comments.
rna1.jpg rna2.jpg

#2 bob1


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Posted 30 November 2011 - 02:25 PM

If you do your extractions well enough you may well see other bands in a RNA extraction, these are also the so called sedimentary (e.g. 28s 18s RNA bands you see normally).

Genomic DNA may show up as bands but usually it is a high molecular weight smear in the top of the gel. Smearing lower in the gel is probably degraded RNA or perhaps mRNA.

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