Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

total RNA Extraction gel control

RNA gel RNA bands

  • Please log in to reply
1 reply to this topic

#1 maegmor

maegmor

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 29 November 2011 - 12:32 AM

I was trying to isolate total RNA from tumor samples and when i ran the extracted samples on gel, i saw 4 bands instead of classic 3 bands which you get to see after total RNA isolation. I believe that the presence of a 4. band is due to the existence of genomic DNA along with total RNA, since i didnt use DNase and some of the samples show up as smears, which i thought is a result of degradation. But i would like to hear your comments.
rna1.jpg rna2.jpg

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,835 posts
415
Excellent

Posted 30 November 2011 - 02:25 PM

If you do your extractions well enough you may well see other bands in a RNA extraction, these are also the so called sedimentary (e.g. 28s 18s RNA bands you see normally).

Genomic DNA may show up as bands but usually it is a high molecular weight smear in the top of the gel. Smearing lower in the gel is probably degraded RNA or perhaps mRNA.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.