Hi all,
I am facing some problem regarding the shrinking of my matrix ie., CM cellulose , upto the packing of column everyting is ok even when i use my buffer to wash the column everything seems to be alright but the moment i load my samples and try to elute it out using a gradient of Nacl solution ,the matrix starts shrinking even upto 1cm after the run is complete when i wash it with my buffer and keep it overnight without running it ,it swells back upto 0.5 cm .Can anyone help me in this regard
Regards,
Neeta .P
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mdfenko
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Excellent
Excellent
Posted 29 November 2011 - 09:33 AM
this is normal. the high salt causes shrinkage of the matrix (some matrices will shrink a lot more).
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
