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What is the maxium amount of glycerol can be added in a PCR reaction?


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#1 oneonenine

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Posted 28 November 2011 - 12:31 PM

If the templates are in glycerol? Thanks.

#2 phage434

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Posted 28 November 2011 - 05:25 PM

Total final concentration of more than about 5-10% will cause problems.

#3 oneonenine

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Posted 01 December 2011 - 09:03 AM

Total final concentration of more than about 5-10% will cause problems.

Thanks. Any method to remove the glycerol?

#4 lamaksha77

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Posted 03 December 2011 - 11:01 AM

well diluting your template is one way to go.......




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