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3t3 cell tured black


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9 replies to this topic

#1 pana

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Posted 28 November 2011 - 04:39 AM

any one encountered such phenomena?
should i defrost new cells?

#2 science noob

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Posted 28 November 2011 - 06:14 AM

any one encountered such phenomena?
should i defrost new cells?


3T3 cells tured *turned* black?

Describe what black is in terms of cell morphology? Is it dark granular spots/dark medium/dark nucleus?

Best solution is if the cells don't look right, they are most probably not right.

#3 pana

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Posted 28 November 2011 - 06:23 AM

medium turned black, granularity in cells and black spots. Morphology of cells did not changed

#4 science noob

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Posted 28 November 2011 - 06:56 AM

What medium did you grow it in?

Granularity in cell cytoplasm indicates unhealthy cells in most cases. Black spots could be infection.
Was the media cloudy or any visible fungal contamination?

#5 pana

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Posted 28 November 2011 - 10:11 PM

Cells grew in DMEM, medium seems clear, thought they started secreting melanin.

#6 science noob

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Posted 28 November 2011 - 10:47 PM

Black DMEM sounds wrong, so I would guess there's definitely something wrong with cells/culture conditions.

#7 pana

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Posted 28 November 2011 - 10:51 PM

like what?

#8 leelee

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Posted 28 November 2011 - 11:41 PM

If I were you, I would thaw a fresh vial, and as long as you don't see the same problem again- just forget about it.

If it is a once of occurrence, its probably a waste of your time to try and find out what went wrong :)

#9 pana

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Posted 28 November 2011 - 11:45 PM

10X

#10 science noob

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Posted 29 November 2011 - 03:20 AM

Have to agree with leelee. Thaw a new vial and use new medium. I still don't understand what black media looks like. Probably best to consult your supervisor if it turns out to be something major/




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