AKTA Injection Syringes
Posted 26 November 2011 - 02:12 PM
We currently use the Superdex 200 10/300 GL column for our gel filtration of HMW proteins. I was wondering how people usually inject their sample into this column. We currently just use a insulin syringe with a removable tip to lock it into the injection port. However we seem to lose some proteins and introduce gas. Can anyone point me to a catalog number of some syringes and protocols that people use?
Posted 27 November 2011 - 01:38 AM
you should have a look at this, its an orginal GE document and they give instructions on sample loading using a syringe.
I don't know how well equiped your machine is (what pumps you have and so on) ...but you may consider using a sample loop or a superloop.
Posted 01 December 2011 - 12:10 AM
Posted 01 December 2011 - 12:12 AM