3 replies to this topic

[Jordan W]

Hello,

We currently use the Superdex 200 10/300 GL column for our gel filtration of HMW proteins. I was wondering how people usually inject their sample into this column. We currently just use a insulin syringe with a removable tip to lock it into the injection port. However we seem to lose some proteins and introduce gas. Can anyone point me to a catalog number of some syringes and protocols that people use?

Thanks,
Jordan

[pDNA]

http://www.mcdb.ucla...s/GESeminar.pdf

you should have a look at this, its an orginal GE document and they give instructions on sample loading using a syringe.
I don't know how well equiped your machine is (what pumps you have and so on) ...but you may consider using a sample loop or a superloop.

Regards,
p

[@bhijit]

I guess its usual to loose some sample when u use the syringe or sample loop -

[@bhijit]

Large sample loop can allow you to dilute the sample and thus the u will loose less protein when loading