Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Lysis buffer volumes of reagents


  • Please log in to reply
No replies to this topic

#1 Sk1

Sk1

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 25 November 2011 - 03:19 PM

I wanted to know what volumes of the reagents do I put together to prepare lysis buffer

Prepare Lysis Buffer using ingredients below:
 
Tris pH 8.0 50mM
NaCl 500mM
EDTA 10mM
SDS  0.2%
Also how much would you add when exteating DNA from maggots, because I carried out the extraction and did obtain DNA however observed it all 'cut up'.
Thanks





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.