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[zee1]

Hi there, this is my first post on this forum, so forgive me if i have put this topic in the wrong place.

I am currently in my third year of university and for a section of my project I need to present a certain antigen on a bacillus subtilis spore as a coat protein. I really need all the help i can get as my molecular biology is not great.
I will display the facts below.

I am trying to present this gene http://www.ncbi.nlm..../nuccore/L07866 on the surface of a bacilus subtilis spore. The region of the genome I need to fuse this sequence to is the 'CotB' region so that it is presented on the surface of the bacillus subtilis spore.
http://www.sciencedi...264410X06008942 this link provides you with a journal of how a recombinant plasmid was made so that tetanus toxin fragment C was presented on the surface of the bacilus subtilis spore. Instead of TTFC i want to present the gene above (invariant surface glycoprotein IVSG)

The general principle around this sort of thing as i understand it are...

Isolate the gene in question

amplify it via PCR

introduce fragment into vector

ensure vector is taken up by organism...

I have a lot of gaps in my knowledge. Please can some one help!
let me add i do not have to do this in real life, just show the process theoretically!

Thank you in advance