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Heavily digested RNA or 5S RNA?

RNA digest 5S RNA degredation

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#1 Alay

Alay

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Posted 23 November 2011 - 10:05 PM

Hi,

This is RNA isolated from a mouse, bone tumor using TRIZOL and a homogeniser. Followed by a cholorform layer extraction.

I have a weird bioanalyzer profile (attached). There is a peak just after the marker. Is this 5S RNA or heavily digested RNA?

If it is 5S RNA, what causes such a large amount to be present in the sampe? I assume it should have a similar ratio to 28S rNA.

If it is heavily digested RNA what causes this?

A clean up column fixes this, however I am wondering how it may have affected my sample?

Cheers,
Alay

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