We are interested in determing if hypoxia/glucose deprivation cause release of calcium from the ER. WE plan to use mag fura2 to measure ER calcium. Two questions I have is there a way to measure calcium in real time, meaning being able to quench the reaction before taking it out of hypoxia, because reoxygenation can have an effect on calcium levels, and how do you harvest the cell after labeling.
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