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Methyl Cellulose for Cell Line Development


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#1 hotstuffdb22

hotstuffdb22

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Posted 23 November 2011 - 05:29 AM

Hello,

I need help preparing methyl cellulose to develop colonies of cells that are mixed with virus for future sub-culturing. Does anyone know the amount of methyl cellulose to use and how to make it?



My goal is to take cells from a mouse's bone marrow, mix with virus, and overlay them on methyl cellulose in 35 mm dishes. However, I'm not sure how much methyl cellulose I need. I believe Methocult is ideal and they recommend 2.6%, but I only have methyl cellulose (Sigma) at the moment. I presume I ought to be able to mix 2.6% methylcellulose in RPMI that is supplemented with 2% FBS, but I'm not sure whether the methycellulose will readily dissolve into the RPMI.



Any help is much appreciated!

Edited by hotstuffdb22, 23 November 2011 - 05:29 AM.





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