Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Methyl Cellulose for Cell Line Development

  • Please log in to reply
No replies to this topic

#1 hotstuffdb22



  • Active Members
  • Pip
  • 28 posts

Posted 23 November 2011 - 05:29 AM


I need help preparing methyl cellulose to develop colonies of cells that are mixed with virus for future sub-culturing. Does anyone know the amount of methyl cellulose to use and how to make it?

My goal is to take cells from a mouse's bone marrow, mix with virus, and overlay them on methyl cellulose in 35 mm dishes. However, I'm not sure how much methyl cellulose I need. I believe Methocult is ideal and they recommend 2.6%, but I only have methyl cellulose (Sigma) at the moment. I presume I ought to be able to mix 2.6% methylcellulose in RPMI that is supplemented with 2% FBS, but I'm not sure whether the methycellulose will readily dissolve into the RPMI.

Any help is much appreciated!

Edited by hotstuffdb22, 23 November 2011 - 05:29 AM.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.