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Reusing SDS-PAGE running and transfer buffers?


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#1 science noob

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Posted 23 November 2011 - 05:08 AM

How many times max. can we reuse both buffers? Some people prefer making fresh every time they do a WB whilst some reuse it countless times.

Does reusing running buffers increase the chances of higher background?

#2 mdfenko

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Posted 23 November 2011 - 09:20 AM

i never reuse running buffers (the page formulation i use has different anode and cathode buffers).

transfer buffer may be reusable a few times but i wouldn't. it may get contaminated by blow through protein, sds from the gel, etc. also, the methanol tends to evaporate so you won't know the concentration after the first run or two.
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