Hello.
To my unfortunate I am forced to measure the intensity of bands on agarose gel after basic PCR.
I found the Scion Image programe in internet, I read the manual, I follow all steps, I think I understand how it should work and no result. It doesn`t allow me to plot even two adjacent bands.
The example in the manual is for PAGE and protein bands, which are situated in column, not in a single row, how it is on the agarose gels.
Does anybody know what could be the problem and if there is a simple, efficient and free software for analysis of DNA bands?
Many thanks,
Nephrite
Measuring of cDNA bands density
Started by Nephrite, Nov 23 2011 04:55 AM
3 replies to this topic
#1
Posted 23 November 2011 - 04:55 AM
#2
Posted 24 November 2011 - 01:41 AM
I've never used (or even heard of) Scion Image, but for what you want you can use ImageJ. Is free online, and very easy to use.
#3
Posted 29 November 2011 - 01:20 AM
Thank you very much!
#4
Posted 02 January 2012 - 04:08 AM
yes you can use ImageJ..
Follow the Link to know more about it
http://lifescienceso...s-software.html
Follow the Link to know more about it
http://lifescienceso...s-software.html
Edited by Biouday, 02 January 2012 - 04:09 AM.














