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Measuring of cDNA bands density


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#1 Nephrite

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Posted 23 November 2011 - 04:55 AM

Hello.

To my unfortunate I am forced to measure the intensity of bands on agarose gel after basic PCR.
I found the Scion Image programe in internet, I read the manual, I follow all steps, I think I understand how it should work and no result. It doesn`t allow me to plot even two adjacent bands.
The example in the manual is for PAGE and protein bands, which are situated in column, not in a single row, how it is on the agarose gels.
Does anybody know what could be the problem and if there is a simple, efficient and free software for analysis of DNA bands?

Many thanks,

Nephrite

#2 almost a doctor

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Posted 24 November 2011 - 01:41 AM

I've never used (or even heard of) Scion Image, but for what you want you can use ImageJ.  Is free online, and very easy to use.

#3 Nephrite

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Posted 29 November 2011 - 01:20 AM

Thank you very much!

#4 Biouday

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Posted 02 January 2012 - 04:08 AM

yes you can use ImageJ..

Follow the Link to know more about it

http://lifescienceso...s-software.html

Edited by Biouday, 02 January 2012 - 04:09 AM.





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