Posted 11 March 2003 - 11:02 AM
Posted 17 March 2003 - 08:33 AM
Posted 18 March 2003 - 09:45 AM
I am just wondering whether I should use AmpliTaq or AmpliTaq gold (the latter needs an 10 min 94 oC activation step). If the two reagents are not thermostable, they might degrade during this extended heating step??
I have been trying to amplify a region of APOE forever! Tried the methods in the literature step by step, still doesn't work (while I never have problems with my PCRs).
The latest methods requires 0.1 ml/L Tween, 100 ml/L DMSO and 20 mM ammonium sulfate.. still not working >