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Primers does not produce any products

RT-PCR CDNA Degenerate primers

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#1 Ritazoye

Ritazoye

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Posted 22 November 2011 - 01:26 AM

Can anyone help me please? I had designed degenerate primers for cytochrome P450 from aligned protein sequences of few available Anopheles CytP450 sequences from NCBI. I had synthesized cDNA as manufacturer's manual (Fermentas #K1641). I had been trying to anneal the primers using different temperatures and time but no avail. Can anyone help me please.





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