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Agar has dried out during cultivation

cloning agar no colonies

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#1 torte

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Posted 22 November 2011 - 12:23 AM

Hi,
I transfected myplasmid into chemically competent cells (E.coli) and let them grow overnight at 37°C, upside down. However, in the morning, there were no colonies, only a thiny plaque and, importantly, the agar was dried out (it was much thinner than yesterday and shrunk).
What can be cause for this?
Thanks a lot
T.

#2 phage434

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Posted 22 November 2011 - 05:01 AM

Very dry air and incubator temperature cause evaporation from the surface of plates. You can make this less of a problem with a pan of water in the incubator, or by putting your plates in plastic bags (or sealing with parafilm).





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