Hi,
I transfected myplasmid into chemically competent cells (E.coli) and let them grow overnight at 37°C, upside down. However, in the morning, there were no colonies, only a thiny plaque and, importantly, the agar was dried out (it was much thinner than yesterday and shrunk).
What can be cause for this?
Thanks a lot
T.
0
Agar has dried out during cultivation
Started by torte, Nov 22 2011 12:23 AM
cloning agar no colonies
1 reply to this topic
#2
phage434
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Posted 22 November 2011 - 05:01 AM
Very dry air and incubator temperature cause evaporation from the surface of plates. You can make this less of a problem with a pan of water in the incubator, or by putting your plates in plastic bags (or sealing with parafilm).
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