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concentrating my sample

crude extract

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4 replies to this topic

#1 tigersgenome

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Posted 21 November 2011 - 05:43 PM

As a part of my work... I am extracting the seeds with various organic solvents. to study the antioxidant activities.

I need to use a rotary evaporator, for concentrating my sample.. But I dont have the option in my laboratory..

so is there any alternative where I could conc my sample, without getting the compounds decomposed.

thanks in advance.

#2 zienpiggie

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Posted 21 November 2011 - 06:49 PM

If you have nitrogen gas, you can stream it under nitrogen gas. If your target compounds are fairly stable, then you may also consider using heating block while streaming nitrogen gas on the surface of your solvents.

#3 science noob

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Posted 22 November 2011 - 12:19 AM

There are commercial columns where you can use to concentrate samples via centrifugation if you know the size of your compound of interest.

#4 mdfenko

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Posted 22 November 2011 - 06:39 AM

you may be able to use a speedvac (or equivalent). with what volumes are you dealing?
talent does what it can
genius does what it must
i do what i get paid to do

#5 tigersgenome

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Posted 25 November 2011 - 06:11 AM

thanks everyone..




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