I am wondering about the "strict caution" about enzyme storing at -20°C. Manufacturers often say that we have to keep enzymes (e.g Taq) upon reception at -20°C. Yet, this enzyme is used in PCR at 95°C for at least 2 h and stay active after alternative cycles of 95/55°C! This means that its stability is very high.
Also, if we look at dishwashers liquid, all enzymes are kept at room temperature and stay active!
So, my question, is my boss right to panic when I forget the Taq on bench for some minutes?
Edited by Biog, 21 November 2011 - 05:23 PM.