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serum deprivation of cell lines


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#1 gnarl

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Posted 21 November 2011 - 01:50 PM

I am stimulating RAW 264.7 macrophages with LPS and then assaying for TNF-a and IL-6. I am new to these types of experiments, so is use of serum free media necessary for this experiment or before the experiment?

#2 zienpiggie

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Posted 21 November 2011 - 06:51 PM

I tried LPS on RAW264.7 for measurement of nitric oxide and I did not starve my cells and it worked fine. So I guess it depends on the purpose of serum starvation in your experiment?




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