Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

serum deprivation of cell lines


  • Please log in to reply
1 reply to this topic

#1 gnarl

gnarl

    member

  • Active Members
  • Pip
  • 14 posts
0
Neutral

Posted 21 November 2011 - 01:49 PM

I am stimulating RAW 264.7 macrophages with LPS and then assaying for TNF-a and IL-6. I am new to these types of experiments, so is use of serum free media necessary for this experiment or before the experiment?

#2 science noob

science noob

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 272 posts
20
Excellent

Posted 22 November 2011 - 12:21 AM

Serum might have some immune factors which could interfere with your assays. Probably do a negative control if you have to include serum, i.e. cells without treatment.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.