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serum deprivation of cell lines

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#1 gnarl



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Posted 21 November 2011 - 01:49 PM

I am stimulating RAW 264.7 macrophages with LPS and then assaying for TNF-a and IL-6. I am new to these types of experiments, so is use of serum free media necessary for this experiment or before the experiment?

#2 science noob

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Posted 22 November 2011 - 12:21 AM

Serum might have some immune factors which could interfere with your assays. Probably do a negative control if you have to include serum, i.e. cells without treatment.

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