I am stimulating RAW 264.7 macrophages with LPS and then assaying for TNF-a and IL-6. I am new to these types of experiments, so is use of serum free media necessary for this experiment or before the experiment?
0
1 reply to this topic
#2
science noob
-
- Active Members
-
- 222 posts
Veteran
12
Good
Good
Posted 22 November 2011 - 12:21 AM
Serum might have some immune factors which could interfere with your assays. Probably do a negative control if you have to include serum, i.e. cells without treatment.
