Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

HELP:Pretreated with inhibitor before 1 h?

inhibitor signal

  • Please log in to reply
2 replies to this topic

#1 note1988

note1988

    member

  • Active Members
  • Pip
  • 7 posts
1
Neutral

Posted 20 November 2011 - 11:17 PM

Hi. Signal pathway inhibitors, such as LY294002 for PI3K/Akt, are often used to explore the role of the signal axis. It's usually described in the paper that "pretreated with LY294002 for 1 h before other treatment", then what's the meaning of the "pretreated for 1 h"?
Should I change the medium after 1 h treatment with the inhibitor and before other treatment, or just add the other treatment without discarding the inhibitor?

Since it's my first topic in the forum, I'm not sure I choose the right field for publication.

#2 GNANA

GNANA

    GNANA

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 209 posts
9
Neutral

Posted 21 November 2011 - 12:53 AM

You got to make sure the pretreated drug action should remain until the end of the time point of the later treated drug..So in this case some drug action would revert in mins to hours once you take it out, so better you pipette out the pretreated medium in a falcon, add the second drug, mix and again add into the flask.. i recommend this rather than wasting another aliquot of drug for the same treatment.
I would prefer being perfectionist rather than a passionist in Research.

I always had an alternate hypothesis....

#3 note1988

note1988

    member

  • Active Members
  • Pip
  • 7 posts
1
Neutral

Posted 22 November 2011 - 10:15 PM

You got to make sure the pretreated drug action should remain until the end of the time point of the later treated drug..So in this case some drug action would revert in mins to hours once you take it out, so better you pipette out the pretreated medium in a falcon, add the second drug, mix and again add into the flask.. i recommend this rather than wasting another aliquot of drug for the same treatment.


Thanks for your help. That's quit helpful. I think maybe I should performed in these two ways, and then detected the targeting gene expression or activation. Therefore, to choose a better method.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.