Posted 28 November 2011 - 07:45 PM
If you're asking about laser lines, well then the 594nm laser is more useful. Not only can it be used to excite fluorochromes like Texas Red and Alexa 594, it can also excite APC and APC-tandems efficiently. So, you can get more bang for your buck with a 594nm. If you're talking about emission maxima, then 594nm and 589nm are a bit too close to differentiate, unless they were the emission maxima of two fluorochromes that were excited by two different lasers on a system with spatially separated beams. In this case, it would be possible to differentiate the two fluors.