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In vitro translation of ER-alpha

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#1 ky_kev



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Posted 20 November 2011 - 03:58 PM

Hi forum,

Just wondering if anyone has any experience in in vitro translating ER-alpha. My vector is pSG5-ERalpha and I'm using T7 RRL Kit from Promega. I've tried with and without E2 without any success. It's not the plasmid or the kit.


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