Posted 20 November 2011 - 03:55 PM
I purified my his-tagged protein using qiagene Ni NTA . i eluted my protein in (50mM NaH2Po4, 300mM NaCl, 250mM imidazole). I will inject this protein to rabbit to make antibody. do I have to get rid of imidazole, if so, should I dialyse it? please help me.
Thank you in advance,
Posted 20 November 2011 - 11:49 PM
Posted 22 November 2011 - 08:52 AM