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Alexa Fluor 594 only showed with blue filter

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#1 candiedhaw



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Posted 20 November 2011 - 01:46 PM

We just got new mouse anti-elastin and Alexa Fluor 594 for donkey anti-mouse.
It is so weird that I can only see nice stained fibers with blue filter, not with yellow filter, where it is supposed to show.
Anyone has any ideal?

Thanks a lot

#2 bob1


    Thelymitra pulchella

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Posted 20 November 2011 - 03:39 PM

I suspect you are either seeing bleed through with your channels, or that you have some form of autofluorescence.

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