Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Alexa Fluor 594 only showed with blue filter


  • Please log in to reply
1 reply to this topic

#1 candiedhaw

candiedhaw

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 20 November 2011 - 01:46 PM

We just got new mouse anti-elastin and Alexa Fluor 594 for donkey anti-mouse.
It is so weird that I can only see nice stained fibers with blue filter, not with yellow filter, where it is supposed to show.
Anyone has any ideal?

Thanks a lot

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,242 posts
336
Excellent

Posted 20 November 2011 - 03:39 PM

I suspect you are either seeing bleed through with your channels, or that you have some form of autofluorescence.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.