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gene expression in E coli with PET21a vector


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#1 mbsea

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Posted 20 November 2011 - 04:53 AM

Hi everyone, I have a problem with my gene and i was wondering if anyone could help me. I am working on a beta glucosidase gene. i want to express it in e coli. i cloned my gene into an amplification vector but i designed my primers that doesn't include the signal peptide of the gene. So there is no start codon in my primers. Do u think must i design new primers or continue to clone it to opet21a and express.

best wishes...

#2 jarannihc

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Posted 20 November 2011 - 06:31 AM

In case of PCR you wouldn't need signal peptide as it would cause misleading results.




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