Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

bacteria - morphology


  • Please log in to reply
2 replies to this topic

#1 Hanka98

Hanka98

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 20 November 2011 - 02:29 AM

Hello anyone,

we isolated bacteria from soil and performed transmission electroscopy of this. Selective staining with uranyl acetate and lead citrate was done and some „toothy“ structures on the surface of the bacteria were detected. Uranyl acetate stained it only diffusionally and weakly, after using of lead citrate significantly granular structure was detected. So has somebody any idea about these structures? I attached also photographs of the bacteria.

Thank you very much for potential advices.

https://picasaweb.go...452409878729970

https://picasaweb.go...454031592030706

Edited by Hanka98, 20 November 2011 - 02:33 AM.


#2 pDNA

pDNA

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 493 posts
14
Good

Posted 20 November 2011 - 09:48 AM

interesting ...looks like vesicles
...but to be honest i have no clue :)

Maybe someone else knows better!

Good luck!
Regards,
p

#3 Julio-Claudian

Julio-Claudian

    i'm unmethylated

  • Active Members
  • PipPipPipPipPip
  • 78 posts
6
Neutral

Posted 10 February 2012 - 02:47 AM

Were you expecting something?

I remember a few years back my when I was observing inclusion bodies in bacteria, similar stuff appeared (though mine looked more like skins peeling off the cell)

>> https://picasaweb.go...feat=directlink
>> https://picasaweb.go...feat=directlink

I found out much later that at different temperatures and growth conditions, biofilm formation will occur. When that happens, it traps matter from the surroundiing that appear as electron-dense objects.

Yours on the other hand look "ordered" to me. It might be something... sorry couldn't be of more help.

Try this:
http://mic.sgmjourna.../2/325.full.pdf

Post back if you discover anything. Would love to know.

Cheers.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.