which gel concentration can separate the 7.5 kDa protein?
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which gel concentration can separate the 7.5 kDa protein?
Started by le_duy211, Nov 18 2011 06:54 PM
6 replies to this topic
#2
bob1
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Posted 19 November 2011 - 01:14 PM
Have a look for Roche Lab FAQs, in the section on proteins, there is a diagram for this sort of thing.
#3
jarannihc
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Posted 20 November 2011 - 06:45 AM
For denatured protein of 7.5KDa try using 15% or 12% SDS-PAGE gels
Edited by jarannihc, 20 November 2011 - 06:48 AM.
#4
pbokiniec
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Posted 21 November 2011 - 03:18 AM
12% wont get the resolution you want.
I'd go 15% gels, run them all the time to get 13, and 16kda proteins.
I'd go 15% gels, run them all the time to get 13, and 16kda proteins.
#5
mdfenko
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Posted 21 November 2011 - 09:27 AM
10% tris-tricine sds page should be good for 7.5 kDa.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#6
le_duy211
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Posted 30 November 2011 - 09:44 PM
I tried using 12% and 15% SDS-PAGE gels, but it didn't work. I will try using 10% tris-tricine SDS-PAGE. Are there anything different between sample buffer used for glycine SDS-PAGE and tricine SDS-PAGE?
#7
mdfenko
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Posted 01 December 2011 - 11:21 AM
i use the same sample buffer for both.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
