designing prirmers with SNPS for diagnostic clinical use
Posted 18 November 2011 - 06:37 PM
How do we find the SNPS
and if we find hem do they have to be redesiggned everytime because what if you can't move the primer?
Posted 22 November 2011 - 09:28 PM
Could you please explain more elaborately on what you are looking for?
I guess for a clinical setting, you would know the SNPs from research papers and also get the primers for them from the same source. The SNP can have three possibilities, AA, AB or BB. So your protocol should be able to tell you, which SNP is present. You could use an ARMS set up, RFLP, or sequencing to figure out what zygosity exists.
NEW!!!! Wonder why this was not part of Chemistry class, ever! on CoffeeTableScience!!!!
Image copyright: Adrian Koh SF.
Replication of this art is strictly prohibited without express permission of the artist