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designing prirmers with SNPS for diagnostic clinical use


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#1 nervous scientist

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Posted 18 November 2011 - 06:37 PM

WHat issues do we have to aware of when desingin SNPS in a diagnostic testting for clinical diaggnosis?

How do we find the SNPS

and if we find hem do they have to be redesiggned everytime because what if you can't move the primer?

#2 Ameya P

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Posted 22 November 2011 - 09:28 PM

NS,

Could you please explain more elaborately on what you are looking for?

I guess for a clinical setting, you would know the SNPs from research papers and also get the primers for them from the same source. The SNP can have three possibilities, AA, AB or BB. So your protocol should be able to tell you, which SNP is present. You could use an ARMS set up, RFLP, or sequencing to figure out what zygosity exists.

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