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Proteomic profiling and mass spectrometry

proteomic protein mass spectrometry

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#1 Junior7

Junior7

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Posted 18 November 2011 - 05:04 AM

Hello All!

Two-dimensional polyacrylamide gel electrophoresis remains a very popular option for global proteomic profiling for its accessibility and low cost. 2-DE has notable drawbacks relative to shotgun proteomics (insufficient resolving power results in multiple proteins per spot, not well-suited to membrane proteins, follow-up by tandem protein mass spectrometry required to ID proteins, etc.).

However, top-down mass spectrometry has not yet advanced to the point where it can compete with 2-DE for discrimination of protein isoforms in most applications. 2-DE, particularly when followed by Western blotting, remains the best option for visualizing isoforms of a given protein and detecting abundance changes in that isoform.





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