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Cloning of IL8-promoter

IL-8 promoter

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3 replies to this topic

#1 Shaun Rouwen

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Posted 18 November 2011 - 03:27 AM

Hi everyone,

I am new to molecular biology and I just started following this class.

However we now have a project on the cloning of the IL-8 promoter out of pDD-Zsgreen1 Reporter plasmid and try to place this into pGL3-Basic Vector.

Now I have 2 problems:

I have to find out, how to digest/cut the IL-8 promoter out of pDD-Zsgreen1. I cannot find the IL-8 promoter sequence and I cannot find the right restriction enzyme to cut this promoter.

Does anybody know where I can find the sequence of the IL-8 promoter, so that I can find the right enyzme to cut this promoter?

2nd problem:
My overal knowledge about Molecular Cloning is very low. Does anybody know a book or website where I can read upon this subject, so that I can make a flying start on the project?

Thanks in advance,

I really need to know these things!

Greets,

Shaun

#2 Rsm

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Posted 18 November 2011 - 03:43 AM

to find the human IL8 promoter
try here: http://www.ensembl.org/index.html
or there: http://genome.ucsc.e...-bin/hgGateway.

Do you have the complete sequence of your plamid?
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#3 Shaun Rouwen

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Posted 18 November 2011 - 04:21 AM

Hey, thanks! i'll take a look at it.

In fact, I dont have the complete sequence of my plasmid yet.
Could I find it somewhere on the internet? I tried 'googeling' it, but couldn't find anything.

#4 Rsm

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Posted 22 November 2011 - 12:54 AM

Google is always a good start. Do you have a "map" of the plasmid, or the correct full name used in publication?
I got soul, but I'm not a soldier




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