Cloning of IL8-promoterIL-8 promoter
Posted 18 November 2011 - 03:27 AM
I am new to molecular biology and I just started following this class.
However we now have a project on the cloning of the IL-8 promoter out of pDD-Zsgreen1 Reporter plasmid and try to place this into pGL3-Basic Vector.
Now I have 2 problems:
I have to find out, how to digest/cut the IL-8 promoter out of pDD-Zsgreen1. I cannot find the IL-8 promoter sequence and I cannot find the right restriction enzyme to cut this promoter.
Does anybody know where I can find the sequence of the IL-8 promoter, so that I can find the right enyzme to cut this promoter?
My overal knowledge about Molecular Cloning is very low. Does anybody know a book or website where I can read upon this subject, so that I can make a flying start on the project?
Thanks in advance,
I really need to know these things!
Posted 18 November 2011 - 04:21 AM
In fact, I dont have the complete sequence of my plasmid yet.
Could I find it somewhere on the internet? I tried 'googeling' it, but couldn't find anything.
Posted 22 November 2011 - 12:54 AM