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Stripping buffer for nitrocellulose membrane

strpping buffer nitocellulose western blotting

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#1 Gaurav S. Choudhary

Gaurav S. Choudhary

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Posted 17 November 2011 - 10:06 PM

Can some body please tell me the receipe for making stripping buffer which can be used on nitrocellulose membrane? Thanks a lot.

Gaurav

#2 Kamran

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Posted 18 November 2011 - 05:21 AM

I use this recipe and strip my nitrocellulose blot for 30 min at 56­ oC with shaking.

100mM β-mercaptoethanol
2% SDS
62.5 mM Tris (pH 6.7)





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