Hi I'm trying to measure ADP binding as a function of flourescence for intrinsic tryptophans. If MANT-ADP is not bound in the active site but is in the solvent will I still get FRET or does it have to be orientated in the active site in order for transfer to occur? I can always measure MANT-directly but its more of a pain since I have to titrate in protein.
Submit your paper to J Biol Methods today!
No replies to this topic