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[cwilsonbran]

Hi I'm trying to measure ADP binding as a function of flourescence for intrinsic tryptophans.  If MANT-ADP is not bound in the active site but is in the solvent will I still get FRET or does it have to be orientated in the active site in order for transfer to occur?  I can always measure MANT-directly but its more of a pain since I have to titrate in protein.

Thanks