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FRET question

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#1 cwilsonbran



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Posted 17 November 2011 - 10:23 AM

Hi I'm trying to measure ADP binding as a function of flourescence for intrinsic tryptophans. If MANT-ADP is not bound in the active site but is in the solvent will I still get FRET or does it have to be orientated in the active site in order for transfer to occur? I can always measure MANT-directly but its more of a pain since I have to titrate in protein.


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