Hi everybody,
Recently I started developing plasmid standard curves for my qMSPs.
I managed to clone the inserts in the vectors and now have vectors in a concentration of app. 4 ug/ul.
To make standard curves, the plasmids schould be linearised to get a good pcr efficiency.
My question is whether anyone has experience with this and if I should linearise just a diluted stock (containing 100000 copies), or start linearising from the 4ug/ul stock.
thanks in advance,
Lise
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