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plasmid standard curves

plasmid standard linearisation

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#1 Lise01

Lise01

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Posted 17 November 2011 - 03:20 AM

Hi everybody,

Recently I started developing plasmid standard curves for my qMSPs.
I managed to clone the inserts in the vectors and now have vectors in a concentration of app. 4 ug/ul.

To make standard curves, the plasmids schould be linearised to get a good pcr efficiency.
My question is whether anyone has experience with this and if I should linearise just a diluted stock (containing 100000 copies), or start linearising from the 4ug/ul stock.

thanks in advance,

Lise





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